Isolation of human eosinophils and their lymphocyte-like rosetting properties.

Abstract

A method for the isolation of large numbers of almost pure human eosinophils from peripheral blood using hypague-ficoll density gradients, gelatin sedimentation and carbonyl iron is described. Eosinophils were obtained from patients with parasitic infections, allergic reactions, undiagnosed eosinophilias and normal volunteers. Erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosettes were formed by a number of eosinophils.