Scavenging effects of natural phenols on oxidizing intermediates of peroxynitrite.

Abstract

Most of peroxynitrite (ONOO-/ONOOH) is formed via the diffusion-limited reaction between nitric oxide and superoxide. In biological systems, the decomposition of ONOO- yields 30-35% of carbonate radical (CO3*-) and nitrogen dioxide (NO2*), which are strongly oxidizing intermediates and are suggested to take a part of the responsibility for the toxicity of nitric oxide (NO*) or ONOO-. Therefore, the current study focuses on the scavenging activities of phenols toward CO3*- and NO2* to protect biomolecules from damage caused by NO* or ONOO- using the technique of pulse radiolysis. From the build-up kinetic of the phenoxyl radicals and the decay kinetic of CO3*- radical, the rate constants of scavenging reactions were determined to be 1.9-3.4 x 10(8) M(-1) x s(-1) and 0.11-1.9 x 10(8) M(-1) x s(-1) for CO3*- and NO2* respectively. The results indicated that the tested phenols are the efficient scavengers of CO3*- and NO2*.