Clonal Propagation of Quercus acutissima Carruth by Somatic Embryogenesis from Embryonic Axes

Abstract

Embryonic axes of Quercus acutissimu were used as explants to initiate somatic embryogenesis in vitro. The addition of 1 mg/l BAP and 1 mg/l GA stimulated secondary somatic embryo production. Mature secondary somatic embryos developed normal shoots when transferred to WPM medium containing 0.1 or 0.5 mg/Z BAP. Root formation occurred upon transfer of these shoots to medium supplemented with 0.5 or 1 mgll IBA. These plantlets possessed the normal chromosome number of 2n = 24.