Genetic polymorphisms in wild and cultured large yellowcroaker Pseudosciaena crocea using AFLP fingerprinting

Abstract

Abstrcat The wild individuals of Pseudosciaena crocea were collected from Guanjing Sea area, East China Sea. The cultured individuals were collected from two cultured populations in two different culture farms in Fujian Province, which were artificially breeding F5-F7. Totally 37 individuals were used. Five primer pairs (E-AAC/M-CTC, E-AAC/M-CAA, E-ACG/MCAG, E-ACG/M-CTT, E-AGG/M-CTG) were used and 503 selectively amplified fragments were detected, ranging from 50 to 450 bp in size, and the number of fragments produced by each pair of primers were 76-155. Among the 503 fragments, 101 bands presented in the total 37 individuals, and 312 bands only presented in part of the wild fish and the cultured, 37 only in the cultured, 53 only in the wild. In the cultured individuals, some fragments presented were not found in the wild individuals. The mean percentages of polymorphic bands of the three populations varied from 69.2% to 76.6% and the mean genetic differences within populations varied from 0.229 9 to 0.246 4, and the maximums all presented in the wild population, indicating the cultured populations had lower polymorphisms. The average genetic distance between the wild and the cultured populations is larger than that between the two cultured populations.