Efficiency of Serological Tests for Detection of Brucellosis in Ruminant at South Provinces of Egypt

Abstract

A total of 2138 serum samples(715 from cattle, 1323 from sheep and 100 from goats) from different districts in Assuit governorate, was tested for the detection of antibodies against Brucella spp. Results obtained by Buffer acidified plate antigen test (BAPAT) and Rose bengal test (RBT) as screening tests indicated a positive reactors percentage of 4.6-5.3, 4.4-7.6 and 10-15% followed by overall brucellosis incidence of 4.5, 5.2 and 5.0 % in case of cattle, sheep and goats, respectively. The Brucella positive reactors were subjected to confirmation by Serum agglutination test (SAT) and Rivanol test (Riv. T) in addition to the previous tests. It confirmed that the BAPAT indicated seroreactors of 91.3 % in sheep and 100% in cattle and goats. Brucella melitensis biotype 3 was isolated from 39 seropositive animals (9 Cattle, 25 sheep and 5 goats). In this investigation, the highest rate of sensitivity (97.4%) was detected by BAPAT, while the highest rate and specificity (87.6%) was by RIV.t.