ANALYSIS OF GENETIC DIVERSITY IN NATURAL GERMPLASM OF LENTINULA EDODES IN CHINA USING RAPD TECHNIQUE

Abstract

Fifty-three wild strains of Lentinula edodes collected from 14 provinces distributed in 8 floristic regions of China were assessed for DNA polymorphism using RAPD technique. A total of 147 RAPD bands were amplified using 10 random primers, and 94% were found to be polymorphic. The variations of RAPD band patterns and DNA similarity among the strains reveal that there is great genetic diversity in the natural germplasm of Lentinula edodes in China. The genetic diversity of the strains in the Hengduashan mountains, the Yunnan Plateau, Taiwan and South China shows much greater. A cluster dendrogram of the sample was constructed using average linkage clustering. Most strains derived from identical or neighbouring regions were classified precedently into the small groups , indicating obvious correllation between division of strains into groups and their localities. The fifty three strains were classified into 4 groups using 0.66 similarity as a cut-off point. GroupⅠand group Ⅱ mainly consisted of strains in the Hengduanshan mountains, the Yunnan Plateau and Central China, while group Ⅲ comprised those in the other regions. Grops Ⅳ was the small branch containing 5 strains derived from North China and Sichuan Province.