Chinese Journal of Indusreial Hygiene and Dccupational Diseases · 2001 · 10 citations · 0 references
PharmacyCell BiologyMolecular BiologyDna RepairMolecular ImagingNucleic Acid BiochemistryComet AssayDna DamageDna Strand BreaksGenotoxicity AssessmentComet Assay ScoringDna ElectrophoresisDna Single-strand BreaksDna Strand Break AnalysisGel ElectrophoresisOlive Tail MomentPeripheral Blood Lymphocyte AnalysisSingle-strand Dna Breaks
Objective To design image analysis system for single cell gel electrophoresis(SCGE). Methods The image analysis soft was designed with Dephi 5.Inhibition of GSH on H 2O 2-induced breakage of single strand DNA in peripheral lymphocyte was detected. Results There were dose-response relationship between H 2O 2 and indexes including length of tail,tail mean,tail Olive moment,tail inertia,tail distributed moment,tail DNA percentage and ratio of tail/head.10 μmol/L of GSH could inhibit H 2O 2-induced breakage of DNA. Conclusion The image analysis software was a good tool for SCGE comet analysis.