[A comparative chromosome map between human and Hylobates hoolock built by chromosome painting].

Abstract

We established chromosomal homologies between all human chromosomes except Y and those of Hylobates hoolock by chromosome painting (chromosome in situ suppression hybridization with human chromosome-specific DNA libraries). Human chromosomes 9,13,15,18,21,22,X libraries hybridized to single G-banding H. hoolock chromosomal region, other human DNA libraries showed at least two homologous segments in H. hoolock chromosomes. 59 segments homologous to one of 22 human autosomes were detected in 18 H. hoolock autosomes. There were at least 39 translocations. Thus an uniquivocally high degree of synteny between human and H. hoolock was confirmed. This will finally allow H. hoolock to be integrated into the overall picture of chromosomal evolution in Hylobatids.