Interleukin-1β overproduction is a common cause for neuropathic pain, memory deficit, and depression following peripheral nerve injury in rodents

TLDR

Chronic pain is frequently accompanied by short‑term memory deficits and depression, which are currently thought to be consequences of the pain itself. The study tests whether overproduction of interleukin‑1β (IL‑1β) in the injured nerve, independent of neuropathic pain, underlies these symptoms in spared nerve injury models. Spared nerve injury upregulates IL‑1β in the sciatic nerve, plasma, and pain‑, memory‑, and emotion‑related CNS regions such as the spinal dorsal horn, hippocampus, prefrontal cortex, nucleus accumbens, and amygdala. IL‑1β overproduction drives pain, memory deficits, and depressive behaviors—effects that are prevented by IL‑1β neutralization or IL‑1R1 deletion and replicated by systemic IL‑1β administration, indicating that IL‑1β, not pain itself, is the common mechanism.

Abstract

Background Chronic pain is often accompanied by short-term memory deficit and depression. Currently, it is believed that short-term memory deficit and depression are consequences of chronic pain. Here, we test the hypothesis that the symptoms might be caused by overproduction of interleukin-1beta (IL-1β) in the injured nerve independent of neuropathic pain following spared nerve injury in rats and mice. Results Mechanical allodynia, a behavioral sign of neuropathic pain, was not correlated with short-term memory deficit and depressive behavior in spared nerve injury rats. Spared nerve injury upregulated IL-1β in the injured sciatic nerve, plasma, and the regions in central nervous system closely associated with pain, memory and emotion, including spinal dorsal horn, hippocampus, prefrontal cortex, nucleus accumbens, and amygdala. Importantly, the spared nerve injury-induced memory deficits, depressive, and pain behaviors were substantially prevented by peri-sciatic administration of IL-1β neutralizing antibody in rats or deletion of IL-1 receptor type 1 in mice. Furthermore, the behavioral abnormalities induced by spared nerve injury were mimicked in naïve rats by repetitive intravenous injection of re combinant rat IL-1β (rrIL-1β) at a pathological concentration as determined from spared nerve injury rats. In addition, microglia were activated by both spared nerve injury and intravenous injection of rrIL-1β and the effect of spared nerve injury was substantially reversed by peri-sciatic administration of anti-IL-1β. Conclusions Neuropathic pain was not necessary for the development of cognitive and emotional disorders, while the overproduction of IL-1β in the injured sciatic nerve following peripheral nerve injury may be a common mechanism underlying the generation of neuropathic pain, memory deficit, and depression.