Complete Sequencing and Analysis of mtDNA in Phlaeoba albonema Zheng

Abstract

The complete mitochondrial genome sequence of Phlaeoba albonema Zheng was determined by using L-PCR and conserved primers walking sequencing. The obtained genome sequence is 15 657 bp in size, containing 13 protein-coding genes, 2 ribosomal RNA and 22 transfer RNA genes. All the 37 genes are conserved in the same orientations as observed in Locusta migratoria. 11 202 bp of the mtDNA are coding for proteins, 1 486 bp for tRNAs, 1 312 bp for rRNA large subunit (lrRNA), and 844 bp for rRNA small subunit (srRNA). The A+T-rich region is 728 bp in size. The genes overlapping sequences are 41 bp in total and are spreading over 9 locations (1—8 bp at each site).A total of 126 bp intergenic spacer sequences are scattered in 21 regions at the size of 1 to 20 bp, where the largest 20 bp region is located between the tRNA~ Lys and ATP8 genes. The predicted secondary structures of both srRNA and lrRNA were compared with that of Ruspolia dubia, and the patterns of base pairs in tRNA anticodon stem and A/T, C/G bias of protein-coding genes in different strands were discussed.