Polymorphisms of the FUT1 gene M307 locus in post-weaning Sutai breed piglet and resistance to F18 fimbrial Escherichia coli in vitro

Abstract

The polymorphisms of FUT1 gene M307 locus in 49 post-weaning Sutai breed piglets were screened by PCR-RFLP,and three genotype were identified as FUT1 M307GG(16),M307AG(19) and M307AA(14).Small intestinal epithelium cells of different genotypes were selected to test the adhesion capability of the wild type E.coli expressing F18ab fimbriae,the recombinant Escherichia coli expressing F18ac fimbriae or the recombinant Escherichia coli secreting and surface-displaying the FedF subunit of F18ab fimbriae,respectively.The results showed that all of wild type and recombinant Escherichia coli were able to adhere to the post-weaning small intestinal epithelium cells of both M307GG and M307AG genotypes.The adhesion capability could be inhibited after incubation bacterium with the corresponding anti-serium.There appeared to be a direct relationship between polymorphisms of FUT1 gene M307 locus in post-weaning Sutai breed piglets and the resistance to pathogenic F18 fimbrial E.coli,which could be determined by adhesion and adhesion inhibition assays in vitro.The M307GG and M307AG genotypes are related to the susceptibility of the PWD and ED,while M307AA related to the resistance.