Efficiency of Thuja orientalis and Artimisia campestris extracts to control of Potato leaf roll virus (PLRV) in potato plants.

Abstract

Potato leaf roll virus (PLRV) was identified by Enzyme-linked immunosorbent assay (ELISA), using PLRV polyclonal antibodies. Extracts from virus infected potato leaves was found to react with PLRV antibodies but not with antibodies of PVY, PVX, PVA and PVM. Extracts from Thuja orientalis and Artimisia campestris at 6 g/L was found to inhibit multiplication of PLRV by 81.72 and 63.6% respectively. Lower effect on the virus (57.8 and 45.7% of inhibition) were observed by the two extracts in plants generated from infected tubers. Extracts application before virus inoculation protected the plants from infection for 10 days by artimisia and 8 days by thuja. No harmful effect was detected on the treated plants as determined by foliage dry weight.