Acta Biologica Szegediensis · 2002 · 15 citations · 3 references
Open access
AgricultureGeneticsPlant SciencesBreeding For Disease ResistancePlant GeneticsPlant Molecular BiologyDisease Resistance GeneticsMolecular Markers In PlantsPlant Disease ResistanceResistance Gene DiscoveryResistance GenesResistant Crop VarietiesDisease Resistance TraitsGenetic Linkage AnalysisGenetic Linkage MappingMolecular Marker ScreeningMolecular Markers For ResistancePlant Disease Resistance GenesPlant Resistance GenesWheat Leaf Rust
The aim of this study was to find molecular markers (RAPD and SCAR) for the wheat leaf rust resistance gene Lr29. Among 81 RAPD primers tested, only one (OPY10) detected an additional band in the resistant NIL of Lr29. The genetic linkage of this molecular marker to Lr29 was tested on a segregating F2 population derived from a cross between the leaf rust resistant line and the susceptible parent GK Delibab. This marker was closely linked to the Lr29 gene. The polymorphic band was cloned and sequenced. Specific primers (SCAR) were synthesized and after amplification only resistant lines showed an amplified product. A second SCAR primer for another Lr29 RAPD fragment (UBC219, Procunier et al., 1995) was also designed and tested.
3
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
John G. Williams, Anne R. Kubelik, Kenneth J. Livak et al. · Nucleic Acids Research · 1990 · 13.2K citations · Full text
Molecular markers and their applications in wheat breeding
P. K. Gupta, Rajeev K. Varshney, P. C. Sharma et al. · Plant Breeding · 1999 · 600 citations