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Characteristics of “Hybrid”-Type Clonal Cell Lines Obtained From Mixed Cultures <italic>In Vitro</italic><xref ref-type="fn" rid="fn1">2</xref>
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1962
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GeneticsPathologyCell CultureCell ProliferationNctc 2472Cancer BiologyTumor BiologyTumor HeterogeneityCancer Cell BiologyFrom Mixed CulturesN2 CulturesCancer ResearchMolecular OncologyHealth SciencesCell DivisionM CellsCell BiologyMalignant DiseaseSomatic Cell GeneticsIn Vitro TechniquesStem Cell ResearchTissue CultureMedicine
In confirmation of previous data, association in vitro of proliferating cultures of high-cancer N1- and low-cancer N2-type cells, derived from NCTC 2472 and NCTC 2555 clonal mouse cell lines, respectively, resulted in the appearance and accumulation of a new type of high polyploid “M” cells. Cells of this type were not observed in Nl or N2 cultures cultivated separately. Clones of M cells were obtained either directly from combined N1 + N2 cultures in vitro or from tumors produced by inoculation of C3H mice with mixed-cell populations. Karyotypes and biological behavior were similar for the 15 clonal M cell lines isolated from both origins. Some morphological and biological traits were common for the M and low-cancer N2 cells, but their malignancy approximated that of N1 cells. The “hybrid” characteristics of M cells remained stable during observation periods of nearly 1 year.