The effect of culture media on the production and measurement of luminol-dependent chemiluminescence.

Abstract

The effect of various media constituents on the production and measurement of luminol-dependent chemiluminescence by neutrophils stimulated by opsonized zymosan was studied. Glucose, calcium ions and magnesium ions were found to be necessary for optimal chemiluminescence although high concentrations of glucose had a detrimental effect. For our luminol-dependent system, a pH of 8.5 give maximal readings. The use of simple balanced salt solutions gave higher chemiluminescence responses than did more complex media with added protein, amino acids, and vitamins. However, the latter were important in the maintenance of optimal cell function.