Molecular phylogenetic analysis of the tonoplast H+-ATPase subunits

Abstract

Vacuolar H+-ATPase is a multi-subunit protein complex, which fulfills a number of crucial functions in plant cell. Different mechanisms are known to be important for the regulation of proton-transporting enzyme activity at transcriptional and post-translational levels. In this investigation we performed a comparison analysis of molecular phylogeny of different subunits of vacuolar H+-ATPase directed in the elucidation of conservative rate of membrane and peripheral complexes of the enzyme. High rate of conservatism was shown for subunits c, d and В, as well as ancient duplications of subunit a.