NONLINEAR RELATION BETWEEN GLOMERULAR HYDROSTATIC PRESSURE AND LOOP OF HENLE PERFUSION RATE

Abstract

A B S T R A C T The present experiments were performed to quantify the effect of changes in distal tubular sodium delivery on glomerular flow dynamics both below and above the normal physiologic range. Glomerular capillary pressure as derived from the tubular stop flow pressure was assessed while the loop of Henle of the same nephron was perfused with varying flow rates. During Ringer perfusion no change of glomerular capillary pressure was observed when flow was increased from 0 to 13 nl/min. Further increasing flow to 27 nl/ min was associated with a reduction of glomerular hydrostatic pressure by an average of 7.0±4.4 cm H20 (+SD). During perfusion at a rate of 43 nVmin glomerular pressure was decreased by a mean of 10.5±4.0 cm H20. Changing the flow rate in small steps revealed that a significant reduction of capillary pressure was found when increasing the flow rate from 13 to 21 nl/ min and that the maximum response was reached at 32 nl/min. No effect of perfusion rate changes on glomerular capillary pressure was observed when 300 mM mannitol was used as perfusion fluid. These results imply that a nonlinear relationship exists between end-proximal