Ubiquitous accumulation of 3′ mRNA decay fragments in <i>Saccharomyces cerevisiae</i> mRNAs with chromosomally integrated MS2 arrays

Abstract

The binding of MS2-GFP protein to arrays of MS2 sites in yeast mRNAs has been used extensively to visualize mRNA localization. We previously reported that arrays of MS2 sites bound by MS2 protein could inhibit Xrn1p and lead to the accumulation of 3' mRNA decay fragments. We suggest that these decay fragments have the potential to complicate mRNA localization studies, as stated in an earlier study.