Electrophoretic studies on human serum albumin

Abstract

Prolonged electrophoresis of human serum has de- monstrated the non-homogeneity of the albumin in phosphate buffer at p1l 8 and ionic strength 0-1, the migration velocities of the boundaries of-the two main components, as apparent from the patterns, differing by about 2% or less (Blix, Tiselius & Svensson, 1941; Hoch & Morris, 1945). Under the conditions ofthese experiments, i.e. similar mobilities and comparatively low ionic strength, the conven- tional methods of analysis of the patterns cannot be applied, since here the separation of the boundary