Anti-inflammatory Activity of Standardised Extracts of Leaves of Three Varieties of Ficus deltoidea

Abstract

Present study aimed to evaluate standardised extracts of different verities of Ficus deltoidea, a traditional medicinal plant, for anti-inflammatory activity using three in vitro assays, lipoxygenase, hyaluronidase and TPA-induced oedema. Methanol and aqueous extracts were standardised by high performance liquid chromatography (HPLC) using two pharmacologically active markers, vitexin and isovitexin, which were isolated from methanol extract of the plant having code FDT1M. The method was validated and then applied to standardise extracts of the plant. In the extracts, the concentration of vitexin and isovitexin varied in the range 2.45 ± 0.00 - 19 ± 0.12 mg/g and 1.58 ± 0.02 - 41.49 ± 0.47 mg/g, respectively. Different extracts of three varieties of the plant displayed different anti-inflammatory activities (p < 0.05). The activity of the extracts was found comparable to apigenin, nordihydroguaiaretic acid, indomethacin, which were used as control (p < 0.05). The results of this study indicate that extracts of leaves of Ficus deltoidea possess antiinflammatory properties.