Electrical pulses and the metabolism of cell-free cerebral preparations

Abstract

The scale of the method limits its accuracy to 5 % in a single determination, which is adequate for determinations of solubilities required for calculating manometer-vessel constants. Greater precision can be obtained by repeated determinations (see Table The special advantage of the present method compared with that of Davidson et al. (1952), lies in the fact that the initial pressure in the vessel is accurately measured, rather than estimated by extrapolation. In addition, temper-ature equilibration is ensured, as preliminary separation of the oil allows time for it to regain the temperature of the thermostat after cooling which occurs during evacuation.