Biological and Pharmaceutical Bulletin · 1993 · 39 citations · 0 references
PharmacyPharmaceutical AnalysisPharmacologyAnalytical ChemistryBiochemical PharmacologyMass Spectrometric AnalysisDrugs Of AbuseInstrumental AnalysisMass SpectrometryToxicological Drug ScreeningAnalytical Characterization Of ConjugatesBovine Serum AlbuminConjugate IdentificationIllicit Drug DetectionMass Spectrometric Drug AnalysisMatrix-assisted Laser Desorption/ionizationPeptide Conjugates
Opium alkaloids (thebaine, codeine and morphine) have been conjugated with bovine serum albumin (BSA) to give individual antigen conjugates which are analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It became clear that 9 molecules of thebaine were contained in a thebaine-BSA conjugate. Codeine and morphine contents in individual conjugates were determined to be 12 and 6 molecules, respectively.