Quantitative analysis of clebopride and its metabolites in rat blood by acid decomposition.

Abstract

6-Chloro-m-anisidine was obtained as one of the products by the acidic decomposition of elebopride and its main metabolites (total CP). This degraded product after derivatized with heptafluorobutyric anhydride was measured by use of mass fragmentography. A good calibration curve was obtained in the range 2-80 ng/ml of clebopride in rat blood. The concentration of total CP in rat blood after intravenous (0.1 and 0.5 mg/kg) and oral (0.5 mg/kg) administration was determined by this method.