Experimental Biology and Medicine · 1983 · 69 citations · 0 references
Veterinary MedicineImmunologyInfectious DiseasesImmunizationVaccinologyVirologyVaccinationVaccine DevelopmentViral HepatitisViral InfectionsViral VaccinesCell-based VaccineExperimental Animal Models Of Viral InfectionHepatitis Virus VaccinationInfectious HepatitisLive Attenuated Vaccine ProductionVaccine Strain Development
Human hepatitis A virus was attenuated in virulence for chimpanzees by passage in FRhK6 and human diploid lung fibroblast cell cultures. A number of variants were developed by passage in cell cultures which showed different levels of virulence/attenuation for chimpanzees. These results were compared to those obtained with marmosets and reported previously. In general, most variants behaved similarly in the two animal types. Two chimpanzees which gave vaccine-like responses following inoculation with HAV cell culture variants were challenged with virulent HAV. Both animals were immune to HAV infection. These findings provide further evidence for the feasibility of developing live, attenuated vaccines against human hepatitis A.