Detection of the Sugarcane Leaf Scald Pathogen,<i>Xanthomonas albilineans,</i>Using Tissue Blot Immunoassay, ELISA, and Isolation Techniques

Abstract

Tissue blot immunoassay, ELISA, and isolation techniques were compared for detection of Xanthomonas albilineans in sugarcane tissues at different positions along the stalk. X. albilineans was detected in the highest frequency in samples from mature plants at the internode superior to 62.5% of the total internodes. This tissue position had the highest frequency of detection and the highest optical density readings with ELISA for both symptomatic and asymptomatic stalks. The number of infected vascular bundles was highest in samples taken from the upper half of symptomatic stalks (.)