The Pollen Spectrum of Apis mellifera Honey from Reconcavo of Bahia, Brazil

Abstract

The use of pollen grains to establish the geographical and botanical origin of honey samples, and for supplying information concerning to the floral class of a honey produced in a region, has been employed since 1895. Pollen analysis has relevant significance for the quality control of a honey that may include numerous pollen grains and elements of honeydew. The objective of this work was to determine the botanical origin of pollen from plants contributing in the composition of honey from the region of Reconcavo of Bahia, Brazil. Methodology: Form each honey sample, a 10 g subsample was diluted in 20 mL of distilled warm water (40C) and centrifuged before the supernatant was drawn out. After centrifugation the pollen sediment was acetolysed following for better observation of the pollen grains. Subsequently, this sediment was mounted in microscopy slides with glycerin jelly for latter pollen grains counting and identification. The identification of the pollen grains found in the samples was determined by comparison with a reference collection from the Palynotheca of the Nucleus of Insect Studies from the Federal University of Reconcavo da Bahia and with descriptions obtained from specialized literature. A total of 70 honey samples of Apis mellifera L. were obtained from beekeepers of 17