Serial Propagation in vitro of Agents Producing Inclusion Bodies Derived from Varicella and Herpes Zoster.

Abstract

SummaryThe inoculation of roller tube tissue cultures of human tissues with vesicle fluid derived from patients with varicella has resulted in the isolation of six cytopathogenic agents. Two of the strains have been maintained for 10 tissue culture passages by employing tissue suspensions as the passage material. Histologically the lesions produced consist of focal accumulations of cells which become swollen, and then degenerate; characteristically, such cells contain intranuclear inclusion bodies. From the eruptive lesions of two cases of herpes zoster, cytopathogenic agents of a similar nature have been isolated and have been propagated serially in cultures of human tissue.