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Quantitation of Collagen Types I and III during Wound Healing in Rat Skin
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1979
Year
Tissue EngineeringEngineeringCollagen TypesPathologyDermatologyOrthopaedic SurgerySkin RegenerationWound CareMatrix BiologyMechanobiologyRat SkinCutaneous BiologyExtracellular MatrixCollagen SynthesisWound HealingMedicineWound ManagementDermal StructureType Iii Collagen
Early wound healing collagen types have not been quantitatively analyzed. The study used Sprague‑Dawley rat back wounds, labeling newly synthesized collagen I and III with tritiated proline, extracting and purifying the collagen, and separating it on an A5‑M column at multiple time points. Type III collagen rose sharply at 10 h, remained elevated through day 12, and returned to a normal 20 % level by 24 h, suggesting it provides early structural support during healing.
SummaryThe collagen Types synthesized during early wound healing have not been quantitatively analyzed. Therefore, open wounds were made on the backs of Sprague-Dawley rats and analyzed at various times for newly synthesized collagen Types I and III. Biopsies were incubated in tissue culture medium with [3H]proline and collagen was extracted and purified prior to separation on an A5-M column. Type III collagen was increased significantly at 10 hr compared to all other times through Day 12. By 24 hr, the percentage Type III collagen returned to a normal skin value of 20%. The early appearance of Type III collagen is associated with an early increase in collagen synthesis and may function in providing initial wound structure and support for subsequent healing events.