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An Analysis of the Wood Sugar Assay Using HPLC: A Comparison with Paper Chromatography
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1984
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Food ChemistryEngineeringCarbohydrate CompositionMedicineFood AnalysisGlycobiologyHemicelluloseAnalytical ChemistryPaper ChromatographyPolysaccharideLiquid ChromatographyFundamental Chemical ResearchPharmacologyWood PulpsChromatographic AnalysisBiomolecular EngineeringChromatographyWood Component
Fundamental chemical research concerning wood and wood-derived products depends on a knowledge of the materials' carbohydrate composition. Separation and quantitation of hydrolyzed carbohydrate components of woods and wood pulps by high performance liquid chromatography is fast and efficient. Sugars are separated with a lead(II)-loaded Aminex cation-exchange resin. In this study, six woods and four pulps were analyzed three times by liquid and paper chromatography (LC and PC). Statistical analysis at the 95% confidence level indicates the two methods are equally accurate for glucan, mannan, and galactan. There is a statistical difference for xylan and arabinan at 95% confidence. The LC precision is better than PC precision for glucan and xylan. The precision is equivalent for arabinan and mannan but not as good for galactan. The differential refractive index detector on the LC is linear up to a loading of 2 mg of glucose. The optimum column operating temperature is 45–55°C.