A Rapid, Simple, Sensitive Method for Measuring Fibrinolytic Split Products in Human Serum

TLDR

The assay’s quantitative results must be interpreted cautiously because fibrin split products are accurately measured, whereas fibrinogen split products are partially absorbed during sample preparation and thus less accurately quantified. The method employs sensitized human group O cells that can be stored at 4 °C for up to three weeks or at ‑20 °C for months, and can be run on Microtiter or Autotiter systems, completing the test in just one hour. The simplified procedure detects fibrinolytic split products as low as 1.0 µg/ml, is more useful for fibrin lysis than fibrinogen lysis, and is especially applicable for diagnosing defibrination syndrome.

Abstract

Summary and ConclusionsA simplified procedure for recognizing and quantitating fibrinolytic split products has been described. It utilizes human group O cells which can be sensitized and stored at 4° for 3 weeks and at ‐20° for many months prior to use. It is very sensitive, detecting 1.0 μg/ml of fibrinogen or split products. Application of the test to the Microtiter and Autotiter systems permits assay of many samples at once. Only 1 hr is required to complete the test and read the results. The interpretation of the quantitative assay result should be carefully made since, while fibrin split products are not absorbed from the sera during the preparation of the sample and are relatively accurately measured, fibrinogen split products tend to be absorbed during sample preparation and are thus less accurately measured. The test would appear to be more useful with fibrin lysis than with fibrinogen lysis and to be especially applicable in the diagnosis of defibrination syndrome.