Experimental Biology and Medicine · 1957 · 27 citations · 0 references
Life SciencesBiochemistryEndocrinologyChromatographic AnalysisHormone BiochemistrySteroid BiochemistryAdrenal EndocrinologyAdrenal HormonesAdrenal Steroid HormonesAdrenal SteroidogenesisSteroid Hormone BiochemistrySteroid MetabolismIsotope DilutionIsotope Dilution AnalysisPaper ChromatographyPlasma Steroid ProfilesQuantitative ChromatographySerum Cortisol MeasurementStable Isotope Dilution AnalysisSteroid Hormone Assays
Summary and ConclusionsA method is presented for measuring Cortisol and corticosterone simultaneously in a single specimen of plasma. The steroids are purified by paper chromatography, eluted and measured by fluorometry. Losses incurred during analytical procedure are compensated by application of an isotope dilution correction. The normal range of corticosterone in human beings is 0 to 5 μtg/100 ml, with a mean of 1.3 ± 2.5 μg/100 ml. The normal range for Cortisol is 4.0 to 17.7 μg/100 ml, with a mean of 10.2 ± 3.6 μg/100 ml.