Glycerolpropylsilane Bonded Phases in the Steric Exclusion Chromatography of Biological Macromolecules

Abstract

Glycerolpropylsilane bonded phases have been found to control the adsorption and/or denaturation of proteins and nucleic acids on controlled porosity glass supports. The bonded-phase thickness is 18-19A while the amount of glycerol moiety varies from 80 to 150 mumoles/g depending on support pore diameter. It has been demonstrated that carbohydrate bonded supports may be used in the chromatography of proteins, nucleic acids, and polysaccharides.