A Technic for the Preparation of Isolated Rat Adrenal Cells

Abstract

SummaryA simple and reproducible technic for the preparation of active isolated rat adrenal cells has been described. Quartered glands are incubated at 37° with mechanical agitation in the presence of trypsin (0.25%). The dispersed cells are collected by centrifugation and suspended in Krebs-Ringer bicarbonate buffer to which has been added glucose (0.2%), albumin (3.0%) and trypsin inhibitor (2 mg/ml). Aliquots of the suspension are incubated at 37° for 2 hr. ACTH in concentrations of 10, 40, and 160 μU increases corticosterone production by the isolated cells. The regression line relating corticosterone production and log dose of ACTH is straight.