Journal of the American Chemical Society · 2011 · 241 citations · 19 references
Life SciencesBiophysicsNeuroscienceCellular NeuroscienceMitochondrial BiologyMolecular NeuroscienceFluorescence MicroscopyFluorescence SpectroscopyMitochondrial Function In NeuronsMitochondrial PhysiologyOptical SpectroscopyPhotophysics Of Fluorescent ProbesEquilibrium Dissociation ConstantTwo-photon Excitation MicroscopyTwo-photon Excitation SpectroscopyTwo-photon Excited FluorescenceTwo-photon Fluorescence ImagingTwo-photon Fluorescence MicroscopyTwo-photon MicroscopyTwo-photon Spectroscopy
We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (Kd(TP)) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+]m in a rat hippocampal slice at a depth of 100−200 μm without interference from other metal ions through the use of two-photon microscopy.
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