Transfer to X-Irradiated Rabbits of Lymph Node Cells Incubated in vitro with Shigella paradysenteriae.

Abstract

Cells were teased from the popliteal lymph nodes of rabbits which had not been injected with dysentery bacilli. These cells were incubated with dysentery bacilli in vitro and transferred to X-irradiated recipients. Agglutinins were detected in the sera of such recipients on the fourth day after transfer. When the cells were heated prior to transfer agglutinins did not appear during the first week after transfer. Similar results were obtained when suspensions of lymph node cells and suspensions of dysentery organisms were injected separately into irradiated rabbits.