Microdetermination and Dynamic Aspects of in vivo Alkyl Lead Compounds

Abstract

A quantitative and qualitative analysis of alkyl lead in the living body is most difficult to determine, therefore, direct microanalization by GC was done.As for stationary phase, 7% Apiezon-L for 4ML, 1.5% SE-30 for 4EL and 1.4-BDS for 3ML and 3EL were more applicable.Glass column and ECD as detector were used.Detection limits reached 1×10-9g. for 4ML and 1×10-11g. for 4EL, 3ML and 3EL.Extractions from biological samples were made using organic solvents after the sample had been acidified using HCl.When more scattered peaks appeared in the extraction solution, 3ML and 3EL were extracted using 1% HNO3 solution and trialkyl lead was transferred into the water layer and re-extracted using organic solvent for purification.