In Vivo Inhibition of Protein Synthesis in Specific Hypothalamic Nuclei by 17 -Estradiol

Abstract

The influence of chronic administration of a pharmacologic dose of 17β-estradiol in adult ovariectomized rats on uptake and incorporation of 14C-lysine into protein was determined for the hypothalamus, cerebellum, and motor cortex, 10, 20, and 30 min after injection of the amino acid. Localization of 14C-lysine incorporated into protein was determined by radioautography for eight hypothalamic nuclei. The data indicate that estradiol inhibits entry of 14C-lysine into the free amino acid pool of the hypothalamus and cerebellum, and inhibits incorporation of 14C-lysine into protein of specfic hypothalamic nuclei, i.e., arcuate, paraventricular, and periventricular.