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Origin of Replication, oriC, of the Escherichia coli K12 Chromosome: Genetic Mapping and Minichromosome Replication

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1979

Year

Abstract

Replication of the circular chromosome of Escherichia coli (Cairns 1964) is bidirectional (Masters and Broda 1971; Bird et al. 1972; Prescott and Kuempel 1972). Replication appears to be initiated at a fixed site (Marsh and Worcel 1977) which has been located about 20 megadaltons (MD) counterclockwise of the ilv operon (von Meyenburg et al. 1977) at 82.5 minutes on the genetic map (Bachmann et al. 1976). For this site, the origin of replication of the chromosome, the symbol oriC has been introduced (Hiraga 1976).