A Miniature Anaerobic Jar for Tissue Transport or for Cultivation of Anaerobes

Abstract

Miniature jars (35-mm. film containers), with acidified copper sulfate and steel wool for obtaining anaerobiosis, were found to be economical and convenient to use for the transport of tissue for anaerobic examination. These jars were compared with Brewer jars and gassed roll tubes. All were equally effective in keeping Bacteroides melaninogenicus and Fusobacterium fusiforme viable for six hours in inoculated tissue. The miniature-jar system can also be used for the cultivation of obligate anaerobes on agar slants or in roll-vials. Alka-Seltzer is an effective source of carbon dioxide