Amphidiploids between Cyclamen persicum Mill. and C.hederifolium Aiton Induced through Colchicine Treatment of Ovules in vitro and Plants.

Abstract

Hybrids of C. pesicumn. diploid and C. hede, rilolium w'ere synt.hesized for interspecific cross-breeding, but were found to be completcly stcrile from the previous paper. Thus, in vitro coichicine treatmont applied to h) brid ovulcs (.C. persicum diploid XC. hederifoliurn ) and colchicine treatmcnt applied to the mature hybrids (C. pelsicurn diploidXC. hedelifoliuln or C. hederifolium X C. persicum diploid)were usod to produced fertile amphidiploids. Many fertile plants were derived from in vitro culture of the ovules t.reated with0.05% colchicine for 10 and 15 days throughout embryogenic callus formation and scveral of the fertile plants were detected to be amphidiploids with a chromosome number of 2 n=82 On the other hand, hybrids were dcrived from in vitro culture of the ovules without colchicinc treat.ment throughout, germination and t.hey werc sterilc plants.5 with a chromosome number of 2 n = 41. Viable ssoods were baroly produced from the maturc hybrids of C. hederifoliuln X C. persicurn diploid which were t.reated with 0.5% colchicine usingtragacanth gum by self-pollination and the flowering plants developed from the seeds were amphidiploids lvith a chromosome number of 2 n=82. Although each of thc amphidiploids induced by thc two methods' and the hybrids exhibitod thc charactc'ristics of the parents, the amphidiploids showed larger flolvcrs and peduncles. than the hybrids and grelv vig'orously. They sholvod high pollen fertility and yielded viable seeds by self-pollination.