Water-Dispersible Superparamagnetic Microspheres Adorned with Two Types of Surface Chains

Abstract

Water-dispersible superparamagnetic polymer/γ-Fe(2)O(3) composite microspheres adorned with two types of surface polymer chains are prepared and characterized. To prepare these spheres, we first synthesize uniform γ-Fe(2)O(3) nanoparticles that are covered by poly(2-cinnamoyloxyethyl methacrylate)-block-poly(acrylic acid) (PCEMA-b-PAA). These nanoparticles are then mixed with a PCEMA homopolymer in CHCl(3) to form an oil phase. The oil phase is dispersed into water under vigorous stirring with the help of two diblock copolymer surfactants, PGMA-b-PCEMA and PSGMA-b-PCEMA. Here PGMA and PSGMA denote poly(glyceryl monomethacrylate) and succinated PGMA, respectively. Solid microspheres with cores composed of PCEMA and PCEMA-b-PAA-covered γ-Fe(2)O(3) nanoparticles are obtained after CHCl(3) evaporation and PCEMA photo-cross-linking. Under certain conditions, the coronal PGMA and PSGMA chains become segregated, thus producing surface bumps, ridges, and valleys. The PSGMA chains preferentially cover the protruding regions. The PSGMA carboxyl groups are used to immobilize bovine serum albumin (BSA). The immobilized BSA retains its activity and binds with anti-BSA. These spheres should be useful in immunoassays.