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A Method for Direct Demonstration of Pleuropneumonia-Like Organisms in Cultured Cells.
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1964
Year
Cell CultureRapid MethodCellular PhysiologyEmbryologyEmbryo CultureUnicellular OrganismPublic HealthCultured CellsPleuropneumonia-like OrganismsDirect DemonstrationCell LinesPplo ContaminationCell BiologyBiologyPathogenesisSlime MouldMicrobiologyTissue CultureMedicine
SummaryDirect microscopic observation of pleuropneumonia-like organisms (PPLO) in cell cultures is easily accomplished following hypotonic treatment, air-drying and staining with orcein. A rapid technique using FL human amnion cells, inoculated with supernatant from suspected cultures, is described. The demonstration of PPLO contamination of 30 cell lines by this rapid method was in complete agreement with results of PPLO-agar techniques.