Effect of Stressful Stimulation on the Incidence of Streptozotocin-Induced Diabetes in Mice1

Abstract

In three experiments, groups of 8-18, 45-day-old male CD-1 mice were injected with a single dose of streptozotocin (STZ) and were followed for 12-31 weeks. Experimental groups were given periodic. light-shock stimulation beginning at various times after streptozotocin injection. Blood samples were taken biweekly from all mice. Blood glucose levels were attenuated over a 22 week period in mice that were stimulated during the first 72 hours after STZ injection. Incidence of insulin-dependent diabetes (blood glucose levels over 150 mg%) was also significantly reduced in stimulated mice. Progressively increasing correlations between sampling order and blood glucose levels, which were significant over the last 12 weeks of one experiment, indicated that blood glucose levels of caged, diabetic mice responded markedly to laboratory procedures.