A Simplified Method for the Quantitative Determinations on Free Pregnanediol Excretion in Pregnancy

Abstract

A rapid, accurate colorimetric method for the quantitative determination of pregnanediol based on the methods of Venning, Talbot and Guterman is described. Serial determinations in normal pregnancy and pregnancy complications have been carried out in over 100 patients. Pregnanediol excretion in the last 28 weeks of the gestation can be used as a quantitative measure of uteroplacental circulation. During this period the placenta is the chief source of this urinary metabolite for corpus luteum activity wanes rapidly after the first trimester. Serial determinations in normal pregnancy and the complications of pregnancy may throw light on the adequacy of the placenta as the essential organ for the survival of the fetus.