Concepedia

Novel fluorescently labeled nucleotides: synthesis, spectral properties and application in polymerase chain reaction

Evgeniy E. Fesenko, A. Yu. Ikonnikova, M. A. Avdonina, Tatyana V. Nasedkina

Mendeleev Communications · 2016 · 15 citations · 22 references

DOI

Concepts

Abstract

The novel C5-modified nucleotides containing indodicarbocyanine dye bearing a carboxyl group at the 3-position of indolenine have been synthesized and incorporated into DNA by commercially available thermostable Taq DNA polymerase. The efficiency of incorporation of labeled nucleotides during polymerase chain reaction was studied in a TB-biochip and THROMBO-Biochip test systems.

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