Isolation and Identification of Aldosterone from Adrenal Venous Blood

Abstract

A highly potent sodium-retaining steroid has been isolated from adrenal venous blood and demonstrated to be identical with aldosterone with regard to: 1) infra-red spectrum, 2) chromatographic behavior of the parent compound and 2 derivatives, 3) biological activity in inducing sodium retention in adrenalectomized rats, 4) ultraviolet absorption and 5) sulfuric acid chromogen.