Wide Linear-Range Detecting Nonenzymatic Glucose Biosensor Based on CuO Nanoparticles Inkjet-Printed on Electrodes

Abstract

Inkjet-printed copper oxide nanoparticles (CuO NPs) on silver electrodes were used to fabricate the nonenzymatic glucose biosensor. The inkjet-printed CuO NPs electrodes produced high and reproducible sensitivity of 2762.5 μAm M(-1) cm(-2) at an applied potential of +0.60 V with the wide linear-detecting range of 0.05-18.45 mM and the detection limit of ~0.5 μM (S/N = 3). The long-term stability and reproducibility of sensor in glucose electro-oxidation resulted from the chemical stability of CuO NPs and pore-like structure formed on Ag surface, which prevented the CuO NPs from conglomeration and the interference of oxygen in the air. Significantly, the effect of interfering species, such as AA, UA, and DA were negligible, whereas sugar derivatives (lactose, fructose, and mannose) show insignificant interference. Finally, the electrode was applied to analyze glucose concentration in human serum samples.