Environment Control for Masspropagation of Tissue Cultured Plantlets (1) Effects of CO2 Enrichment on the Plantlet Growth during the Multiplication Stage

Abstract

Plantlets of Statice (Limonium Hybrid) were tissue-cultured in glass test tube with plastic-formed cap (the number of air changes per hour: 1.5hr-1) for multiplication under higher CO2 gas concentration and higher photon flux (150μmol m-2sec-1, 400-700nm) conditions than normal culture conditions. Concentration of CO2 gas outside the vessel during the light period, containing the plantlet, was maintained at about 950vpm to increase the inside concentration. The inside concentration during the light period decreased day by day to 200-400vpm at 50 days after treatment, showing the photomixotrophic growth of the plantlets. The growth of the plantlets was promoted by CO2 enrichment under higher photon flux.