Journal of Economic Entomology · 1972 · 21 citations · 0 references
Life SciencesBiochemistryNeuroscienceEnzymologyMolecular NeurosciencePesticide ToxicologyAtp HydrolysisEnzyme CharacterizationEnzyme InhibitionEnzyme KineticsNeurotransmissionSynaptic TransmissionAdenosine TriphosphatasesAtp Hydrolysis MechanismChlorinated HydrocarbonsChlorinated SolventsEnzyme ActivityEnzyme Activity RegulationSodium-potassium Atpase
Adenosine triphosphatase (ATP phosphohydrolase, E. C. 3.6.1.3) activity which is sensitive to DDT as compared with a noninsecticidal analogue DDE has been found in the synapses of rat brain. Inhibition of the Mg+-, Na+-, and K+-stimuated enzyme component by DDT required a particular combination of the cations which did not favor optimal Na+–K+ ATPase activity. The system exhibited a negative temperature correlation in DDT sensitivity. The I50 of the Na+ and K+ activated component against DDT was 10-6 M, and the system was relatively insensitive to other chlorinated hydrocarbon insecticides. A portion of the enzyme activity could be “solubilized” by ultrasonication and eluted as one peak on Sephadex columns together with bound 14C-DDT.