Mechanism of Formation of Hexosemonophosphate in Muscle and Isolation of a New Phosphate Ester

Abstract

In minced and washed frog muscle incubated in phosphate buffer, added adenylic acid transfers inorganic phosphate to carbohydrate resulting in the formation of hexosemonophosphate. The first phosphorylation product proved to be a new ester which was isolated as the crystalline brucin salt and had the properties of glucose-1-phosphoric acid; when added to frog muscle extract it was converted in a few minutes to the Embden ester.